Daily Calcs

Molarity Calculation

Find the mass required to prepare a solution of known volume & concentration, find the volume of solution required to dissolve a known mass to a specific molarity or find a concentration of a solution resulting from a known mass & volume.

Dilute a Stock Solution

Determine the required volume from a stock solution of known concentration to make a final solution of desired volume and concentration.

Formula Weight

Computes the average molecular weight (MW) of molecules by entering the chemical formula (i.e. C3H2O4). Supports complex molecules such as "C3H2(NO)4" and "C3H2Cl4.(H2O)3".

Formula Weight calculate molar solution
1.000g =
1 mmol =

Atom Subtotal Mass (%)

Cell Culture Reference

Select a vessel to see useful numbers such as surface area, seeding density, growth media volumes and more.

Surface Area (mm²)
Seeding Density
Cells at confluency¹
Growth medium (ml)
Versene (ml of 0.53 mM EDTA)
Trypsin (ml of 0.05% trypsin 0.53 mM EDTA)

¹ The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used.

Transfection Calculator

LIPOFECTAMINE

2000

LTX

RNAiMax

	What is the plate format* you will be using? *Refer to plate format code to enter correct value according to your plate format.
	How many replicates?
	Recommended* Lipofectamine�2000 dose:
	*Optimum Lipofectamine� 2000 dose should be determined for each specific cell line using quick protocol due to the variation between different cell lines. If a dose that varies from the reccommended dose is desired enter the value in the highlighted cell.	µl/well
	How many Different DNA* samples to test? *DNA stock solutions should be between 0.5-2 ug/ul
	Recommended dose* of DNA per well:
	*If a DNA dose different from the recommended dose is desired enter the value in the highlighted cell.	ng/well
	Volume of Lipofectamine� 2000/DNA dilution to add per well:

Description	Process A	Process B
Basal Medium ($/L)
Supplement 1 ($/L)
Supplement 2 ($/L)
Supplement 3 ($/L)
Supplement 4 ($/L)
Supplement 5 ($/L)
Supplement 6 ($/L)
Supplement 7 ($/L)
Supplement 8 ($/L)
Supplement 9 ($/L)

Description	Process A	Process B
Basal Medium ( L )
Supplement 1 ( L )
Supplement 2 ( L )
Supplement 3 ( L )
Supplement 4 ( L )
Supplement 5 ( L )
Supplement 6 ( L )
Supplement 7 ( L )
Supplement 8 ( L )
Supplement 9 ( L )
Total Batch Size (L)	0.000	0.000

Description	Process A	Process B
Price of materials used
Grams of Protein Produced
Cost per gram of protein produced

	What is the plate format* you will be using? *Refer to plate format code to enter correct value according to your plate format.
	How many replicates?
	Recommended* Lipofectamine�RNAiMax dose:
	*Optimum Lipofectamine�RNAiMax dose should be determined for each specific cell line using quick protocol due to the variation between different cell lines. If a dose that varies from the reccommended dose is desired enter the value.	µl/well
	How many Different siRNA* samples to test? *siRNA working solution should be at 10uM (10pmoles/ul).
	Recommended dose* of siRNA per well:
	*If a siRNA dose different from the recommended dose is desired enter the value.	pmoles/well
	Volume of Lipofectamine� RNAiMax/siRNA dilution to add per well:

	What is the plate format* you will be using? *Refer to plate format code to enter correct value according to your plate format.
	How many replicates?
	Recommended* Lipofectamine�LTX dose:
	*Optimum Lipofectamine� LTX dose should be determined for each specific cell line using quick protocol due to the variation between different cell lines. If a dose that varies from the reccommended dose is desired enter the value.	µl/well
	How many Different DNA* samples to test? *DNA stock solutions should be between 0.5-2 ug/ul
	Recommended dose* of DNA per well:
	*If a DNA dose different from the recommended dose is desired enter the value.	ng/well
	Volume of Lipofectamine� LTX/DNA dilution to add per well:

Day	VCD (million cells/mL)	Titer (mg/L)