Molarity Calculation

Find the mass required to prepare a solution of known volume & concentration, find the volume of solution required to dissolve a known mass to a specific molarity or find a concentration of a solution resulting from a known mass & volume.

Calculate the Molartity of a solution

grams/mole

milligrams

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Dilute a Stock Solution

Determine the required volume from a stock solution of known concentration to make a final solution of desired volume and concentration.

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Formula Weight

Computes the average molecular weight (MW) of molecules by entering the chemical formula (i.e. C3H2O4). Supports complex molecules such as "C3H2(NO)4" and "C3H2Cl4.(H2O)3".

Calculate the molecular weight of a formula

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Formula Weight
calculate molar solution
1.000g =
1 mmol =
Atom Subtotal Mass (%)

Cell Culture Reference

Select a vessel to see useful numbers such as surface area, seeding density, growth media volumes and more.

Find information about a specific vessel

Surface Area (mm2)
Seeding Density
Cells at confluency1
Growth medium (ml)
Versene (ml of 0.53 mM EDTA)
Trypsin (ml of 0.05% trypsin 0.53 mM EDTA)

1 The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used.

Distance

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Speed

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Area

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Volume

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Weight

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Temperature

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Molarity

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Fed-Batch Economic Calculator

Harvested Protein
Concentration (g / L)

Process A Process B

Economy Calculator

Cost of materials used

Description Process A Process B
Basal Medium (\$/L)
Supplement 1 (\$/L)
Supplement 2 (\$/L)
Supplement 3 (\$/L)
Supplement 4 (\$/L)
Supplement 5 (\$/L)
Supplement 6 (\$/L)
Supplement 7 (\$/L)
Supplement 8 (\$/L)
Supplement 9 (\$/L)

Economy Calculator

Quantity used to produce protein

Description Process A Process B
Basal Medium ( L )
Supplement 1 ( L )
Supplement 2 ( L )
Supplement 3 ( L )
Supplement 4 ( L )
Supplement 5 ( L )
Supplement 6 ( L )
Supplement 7 ( L )
Supplement 8 ( L )
Supplement 9 ( L )
Total Batch Size (L) 0.000 0.000

Economy Calculator

Result

Description Process A Process B
Price of
materials used
Grams of
Protein Produced
Cost per gram of
protein produced
Specific Productivity Calculator

Specific Productivity Calculator

Duration of culture in days
Transfection Calculator

Transfection Calculator

LIPOFECTAMINE

2000
LTX
RNAiMax
What is the plate format* you will be using?
*Refer to plate format code to enter correct value according to your plate format.
How many replicates?
Recommended* Lipofectamine™2000 dose:

*Optimum Lipofectamine™ 2000 dose should be determined for each specific cell line using quick protocol due to the variation between different cell lines. If a dose that varies from the reccommended dose is desired enter the value in the highlighted cell.
µl/well
How many Different DNA* samples to test?
*DNA stock solutions should be between 0.5-2 ug/ul
Recommended dose* of DNA per well:

*If a DNA dose different from the recommended dose is desired enter the value in the highlighted cell.
ng/well
Volume of Lipofectamine™ 2000/DNA dilution to add per well:

Transfection Calculator

Result